Digesting vector MINV-IRES-mB7-2 by enzyme MuntI and BamHI to obtain IRES-mB7-2 gene fragment with ambly end and BamHI sticking end.

• 2MunI酶切载体MINV-IRES-mB7-2,末端钝化,然后BamHI再酶切,得到含钝末端和BamHI粘末端的IRES-mB7-2基因片段;