DNA sequencing result showed that the terminative code of nm23-Hl gene in pLXSN-nm23-H1-EGFP has been mutated from ATG to TGA. The fusion protein of nm23-Hl-EGFP is stablely, continuously and high efficiently expressed in transfected L9981 cell line.

• 应用质粒DNA的转化，酶切，连接技术成功构建了逆转录病毒真核表达载体pLXSN一nm23一Hl一EGFP，将质粒pLXSN一nm23一HI一EGFP转染L9981细胞株后，nm23一Hl一EGFP融合蛋白能够在受染细胞内持续，稳定和高效表达。