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- Objective To seek an effective way to kill gastric cancer cells by using cytosine deaminase (CD) gene. 目的探讨胞嘧啶脱氨酶基因对胃癌细胞的杀伤作用。
- They expanded the number of mesenchymal stem cells in the laboratory and then used a retroirus ector to insert the gene cytosine deaminase into the cell. 他们在实验室内扩增间充质干细胞数量，然后利用逆转录病毒载体将胞嘧啶脱氨酶基因插入细胞基因组中。
- Methods Recombinant expression vectors containing CD (cytosine deaminase)and/or TK(thymidine kinase) gene under CMV promoter were constructed successfully. The vectors were transfected to GLC-82 tumor cell lines by use of lipofectamine. 方法 分别构建了以 CMV为启动子 ;含单纯疱疹病毒胸苷激酶（ HSV-TK）和 /或大肠杆菌胞嘧啶脱氨酶（ Ecoli.;CD）单、双自杀基因的真核表达载体。
- Methods:The HIF-dependent recombinant adenovirus Ad-5HRE/hCMVmp-BCD was constructed by DNA recombinant technique. Western blot was used to detect HIF-dependent expression of bacterial cytosine deaminase (BCD). 方法借助DNA重组技术构建HIF依赖性表达的重组腺病毒载体Ad-5HRE/hCMVmp-BCD。
- cytosine deaminase gene (CD gene) 胞嘧啶脱氨酶基因
- Keywords Cholangilcarcinoma;Thymidine kinase;Cytosine deaminase;Gene therapy; 胆管癌;胞嘧啶脱氨酶;胸苷激酶;基因疗法;
- Observation on gene augmentation of cytosine deaminase to neural stem cells and its expression in vitro 离体实验条件下胞嘧啶脱氨酶基因修饰神经干细胞及其表达的观察
- Efficacy of antitumour colon cancer cell vaccine modified by eschrichia coli cytosine deaminase gene 胞嘧啶脱氨酶基因修饰的结肠癌细胞瘤苗抗肿瘤研究
- The Relationship between the Bystander Effect of E. Coli. Cytosine Deaminase Gene Depends and the Immune Status of Host 胞嘧啶脱氨酶基因旁观者效应与宿主免疫状态关系
- Study of neural stem cells modified by cytosine deaminase gene and its expression in vitro 胞嘧啶脱氨酶基因修饰神经干细胞及其表达的离体实验研究
- X-ray combined with cytosine deaminase suicide gene therapy enhances killing of colorectal carcinoma cells in vitro X线联合胞嘧啶脱氨酶基因对结肠直肠肿瘤细胞的杀伤作用
- Inhibitory Effect in vivo of Recombinant Adenovirus Containing Cytosine Deaminase Gene on Tumors 含胞嘧啶脱氨酶基因重组腺病毒体内抑瘤作用的研究
- Killing Effect of Cytosine Deaminase and HSV-tk Double Suicide Genes on Lymphoma Cells Mediated by Retrovirus Vector 逆转录病毒介导的双自杀基因对淋巴瘤细胞的杀伤作用研究
- Targeting Antitumor Effect of Cytosine Deaminase Gene on Human Colon Carcinoma Implanted in the Nude Mice 胞嘧啶脱氨酶对表达不同水平癌胚抗原的大肠癌细胞株的杀伤作用
- Experimental study on human pancreatic carcinoma treated by cytosine deaminase gene therapy 胞嘧啶脱氨酶基因治疗人胰腺癌的实验研究
- LETHAL EFFECT OF 5 FC ON MAMMALIAN CELLS GENETICALLY MODIFIED TO EXPRESS CYTOSINE DEAMINASE 5-FC对经基因修饰而表达胞嘧啶脱氨酶的哺乳类细胞杀伤作用
- Experimental Study on Treatment of Colon Cancer with E.Coli. Cytosine Deaminase Suicide Gene 胞嘧啶脱氨酶自杀基因治疗大肠癌的实验研究
- Expression of Cytosine Deaminase Gene in Human Colon Carcinoma Cells by Recombinant Retroviral Vector 逆转录病毒介导CD基因在人结肠癌细胞中表达
- Keywords recombinant adenovirus;gene therapy;cosmid;cytosine deaminase;thymidine kinase; 重组腺病毒;基因疗法;粘粒;胞嘧啶脱氨酶;胸苷激酶;
- Keywords Gene therapy;Radiotherapy;Cervix neoplasms;Cytosine deaminase;Fluorouracil; 基因疗法;放射疗法;宫颈肿瘤;胞嘧啶脱氨酶;氟尿嘧啶;
