Cx36 also bound to PDZ1 domain of ZO-1 in mouse retina and Cx43 bound to PDZ2 domain of ZO-1 in mouse brain. Fusion protein was detected in the membranes with anti-GST and coomassie blue.

• 与鼠视网膜组织孵育免疫印迹显示Cx36与ZO-1的PDZ1结构域结合,而对照组Cx43与ZO-1的PDZ2结构域结合,相同膜洗脱后抗GST抗体和考马斯亮蓝染色显示GST-PDZ融合蛋白表达。