Cultured monolayer-confluent human RPE cells were scraped with a trephine and a cotton stick, and set up the injured model of RPE cells with round scraped area.

• 利用体外培养的单层近融合期人RPE细胞，采用棉签和角膜移植用环钻做圆形细胞刮伤区，建立体外RPE细胞损伤模型。