Con clu sion:Sonication-Urea-CHAPS-DTT-SB 3-10 can improve the2-DE resolving power and increase the accuracy of protein identification by bio-mass spectrometry.

• 结论:超声兼Urea-CHAPS-DDT-SB3-10菌体蛋白提取法提高了双向电泳的分辨率,进而可增强生物质谱鉴定蛋白质的准确度。