Chinese challenge strain F48E8 of newcastle disease virus(NDV) was papagatedin the allantoic cavity of 10-day old embiyonated SPF chicken eggs.

• 以国内新城疫病毒（Newcastle disease Virus，NDV）标准强毒株F_(48)E_8接种10日龄SPF鸡胚，收集死亡鸡胚尿囊液经蔗糖密度梯度离心后获得较纯的病毒。