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- GHR基因5’端Promoter区 growth hormone receptor(GHR)gene 5' promoter region
- 扩增p 16 promoter一luc一pGLZ一Basic报告基因表达质粒并提取其DNA。 P16-promoter-luc-pGL2-Basic plasmid DNA was prepared after plasmid amplification, and digested by EcoR I and Hind III.
- F-actin F-actin
- β-actin β-actin
- actin基因 actin gene
- α-SM-actin α - smooth muscle - actin
- U3R调控区与pEGFP-N载体融合构建了鸡CES细胞特异表达载体pEGFP-cENS2-Promoter,通过转染胚盘细胞、CEF细胞验证其特异的启动子活性。 The CMV promoter was cut off from pEGFP-N1, and the putative U3-R fragment was inserted just upstream of GFP gene. Results of restriction digestion and DNA sequencing showed that the vector pEGFP-cENS2-Promoter for expressing in CES cell was successfully constructed.
- actin结合活性 actin binding activity
- 为此，本文构建了植物表达载体pZPZ 12一rd29A promoter一ABPg和pZP212一rd29A promoter一ABPg一GFP，并成功获得了转基因的拟南芥植株。 To analysis the expression of ABP9 in transgenic plant, the plant expression vectors pZP212-rd29Apromoter-ABP9 and pZP212-rd29Apromoter-ABP9-GFP was constructedBy means of Agrobacterium tumefaciens -mediated, two constructs were transformed into plant then obtained transgenic plant.
- β-actin基因 β-actin gene
- -βactin启动子 β-actin promoter
- 接着,用Promoter Proscan Version 1.7软件进行基础启动子分析,发现两段启动子序列的基础启动子区均在在MOC1基因起始密码子ATG前639bp-389bp之间。 We analysised the core promoter region with software of Promoter Proscan Version 1.7, the result indicated that the two fragment have core promoter region in 639bp to 389bp before initial code ATG of M0C1 gene.
- 鸡β-actin启动子 Chicken β -actin promoter
- EMA、Actin、S-100、ER阴性; Negatived for EMA,Actin,S-100,ER;
- 标记F-actin,荧光显微镜下观察。 F-actin was labled and examined using fluorescence microscope.
- 高糖组F-actin荧光强度为对照组的44.5%； High glucose can decrease the fluorescent intensity of F-actm to 44.5%25 (p<0.01);
- MOMA-2表达增加,SM-actin表达降低(P<0.05)。 Even,upregulation of MOMA-2 and downregulation of SM-actin were also detected in latter(P<0.05).
- 高速电视摄影管
- 电离辐射对血管内皮细胞骨架蛋白F-actin影响的激光共聚焦显微镜观察 The Observation of the Influence of Ionizing Radiation on the Image of F-actin in Vascular Endothelial Cells with Confocal Laser Scanning Microscope
- 结果表明,LIM结构域2在hhLIM与actin相互作用及调节actin细胞骨架组构中起决定性作用. In conclusion, LIM domain 2 at the C-terminus of hhLIM plays a central role in F-actin polymerization and cytoskeleton stabilization, whereas the first LIM domain is essential for the nuclear localization of hhLIM.
