Cell culture and amidase induction were further performed under different conditions, and the highest amidase activity of 80U/L was harvested in E. coli TB1/pMAL-Ami.

• 在不同培养和诱导条件下对酰胺酶基因进行了诱导表达，在E.；coli TB1/pMAL-Ami 中获得了80U/L的酰胺酶酶活。