CS-3000 and PLUS blood cell separate machine were used to collect peripheral blood stem cell. Procedure Lower Temperature instrument was used to lower MNC to subzero 80 celsius degree and stored in subzero 196 celsius degree liquid nitrogen.

• 用CS-3000,Plus血细胞分离机采集外周血造血干细胞,将分离的单个核细胞 (MNC)经程序降温仪降至 -80℃后,冻存 -196℃液氮。