By using long-distance inverse PCR, Arg (cgt) and Arg (egg) in the expression fragment of phytase phyA gene (GenBank : accession numbers AF218813 and AAF25481) were mutated synonymously to Arg (aga), which is a bias code of yeast.

• 对来源于本课题组自主分离的黑曲霉N25(Aspergillus niger China Strain)的植酸酶基因phyA(GenBank:基因注册号为AF218813,蛋白质序列号为AAF25481)进行PCR介导的定点突变。