By using fluorescence staining method, the effect of receptor-mediated endocytosis on intracellular pH (pHi) of mouse macrophages was studied with adherent cell analysis and sorting cytometer 570 (ACAS 570).

• 用pH值敏感荧光探针Snaflcalcein－AM标记体外培养48小时的小鼠腹腔巨噬细胞，570型粘附式细胞仪观察巨噬细胞在对刀豆素A进行受体介导内吞过程中细胞内pH值的变化。