By the means of leave assay of hygromycin resistance, PCR amplification of a HPT fragment and Southern blot, it showed that RCOI and OsAOS fragments were successfully inserted into the rice genome.

• 利用离体叶片潮霉素快速鉴定、潮霉素PCR和Southern杂交等方法对转基因植株后代进行鉴定，证明了RCOI和OsAOS基因的片段正确插入水稻基因组；