By the inactivated protoplast fusion method carrided out, fusants was selected through the means by TTC,and the fusant RHZ-7 was obtained which can produce 9.27% (v/v) alcohol and 0.721g/L ester.

• 采用紫外线灭活MT-14原生质体后与呼吸缺陷型突变株HX-9进行原生质体融合；在再生培养基平板上挑选TTC反应呈红色的融合子；并经过分级筛选；得到一株产酒精达9.；27%25(v/v)、产酯0