By response surface experiment, it was found that the combination use of 0.54% PEG6000, 0.62% trehalose and 0.31% DTT could effectively improve the PAL activity by 92.9%.

• 响应面实验发现；海藻糖、聚乙二醇6000、二巯基苏糖醇在实验浓度范围内交互作用很小；0.；62%25海藻糖、0