By optimizing the parameters of co-culture between EHA105 (pBI121-IN) and cotyledonary node of soybean Ji30, we established a effective Agrobacterium EHA105-mediated genetic transformation system of cotyledonary node of Ji30.

• 用EHA105(pBI121-IN)工程菌液侵染大豆“吉30”子叶节，通过优化EHA105(pBI121-IN)对“吉30”子叶节遗传转化的各种参数指标，建立了农杆菌EHA105介导的大豆“吉30”子叶节遗传转化体系。