By immunohistochemical study, the bone morphogenetic protein(BMP) content in callus was calculated by computerized interacive morphometry (CIM) in the 1st, 2nd, 3rd, 4th, 5th week after fracture.
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于骨折后第1,2,3,4及5周用断颈法每次每组处死5只兔,取骨痂进行抗骨形态发生蛋白(BMP)单克隆抗体免疫组化染色,并用CMIAS型图像分析仪对BMP含量进行定量分析。
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