But most of the drugs has tew fluorescence quantum yield, so some reagents must be added to produce fluorescence.

• 间接测定法是由于大部分药物本身无荧光或者在水溶液中的荧光量子产率低，无法直接测定药物荧光，必须通过加入其它试剂提高药物的荧光量子产率而建立的荧光分析法。