Brain-stems of 52 Wistar rats were stabbed with steel needle and the changes of GFAP (Glial Fibrillary Acidic Protein) positive astrocytes as well as VIM (Vimentin)positive astrocytes were demonstrated with LSAB immu noh istoc he mistry.

• 52只Wistar大白鼠针刺致脑干机械性损伤，LSAB法显示胶质原纤维酸性蛋白阳性（GFAP+）星形胶质细胞和波形蛋白阳性（VIM+）星形胶质细胞改变。