Bovine serum albumin(BSA) and ovalbumin(OVA) were used as protein carriers respectively to couple with hapten ENR by an active ester method, and complete antigens ENR-BSA and ENR-OVA were prepared.

• 通过N-羟基琥珀酰亚胺活性酯法(NHS法)将半抗原ENR分别与蛋白质载体牛血清白蛋白(BSA)和卵清蛋白(OVA)偶联成为全抗原ENR-BSA和ENR-OVA。