Both the constructed plasmids pSMLFPGA and pKKFPGA contained the PGA gene ,trc promoter and rrnB transcript terminator, differ in the replicon and antibotic marker, pKKFPGA contained multicopy replicon(COLE 1) and ampicillin marker .

• 本文将粪产碱杆菌青霉素G酰化酶(AfPGA)基因构建表达载体pKKFPGA和pSMLFPGA，pKKFPGA和pSMLFPGA均含trc启动子、青霉素G酰化酶基因、rrnB转录终止子，其中pKKFPGA含有氨卞青霉素抗性基因和colE1高拷贝复制子；