Biomarkers of replicative senescence were assess in this model. The averages of life span of IMR 90 under NG,LG and HG culture conditions were 55 0,58 3 and 47 2 population doubling levels(PDL),respectively.

• 低、正常和高浓度葡萄糖培养条件组IMR 90细胞平均寿限分别为 5 8 3、5 5 0和 4 7 2PDL(群体倍增水平 ) .