Bi-Bi reaction model,namely Ordered Bi-Bi mechanism,was proposed and was compared with traditional ping-pong Bi-Bi mechanism adopted to enzymatic transesterification usually.

• 125脂肪酶为催化剂,甘油三油酸酯和甲醇为底物,采用有序机制模型对酶促合成生物柴油的酯交换反应动力学进行了研究,并与经典乒乓机制模型进行了比较。