Best rooting was observed on MS supplemented with IBA 0.2 mg L-1. After 5-day treatment with colchicine, 40.0% of M. oleifera regenerants and 36.7% of M. stenopetala regenerants were found chromosome doubling.

• 用不同浓度秋水仙碱对染色体进行加倍诱变；以0.；2%25秋水仙碱处理5d；加倍效果最好；印度辣木达到40