Based on the experiment,the enumeration method of Bifidobacterium by rectangular test tube was put forward,according to the anaerobic specificity of double layer half solid culture.

• 利用双层半固体培养基具有良好厌氧性的特性,在实验的基础上,提出了采用方形试管对双歧杆菌进行计数的方法,介绍了该方法的基本原理和操作步骤。