Based on the cloning of their parts and the analysis of homology of their genetic nucleotide sequence and deduced amino acid sequence, primers of internal standard DNA of gene AcrA. and AcrE were designed and synthesized. The internal standard of AcrA.

• 在克隆AcrA、AcrB部分基因片段并进行基因及编码蛋白的同源性分析的基础上,设计合成构建AcrA、AcrB的内标准DNA所需引物,经过3次(12个)PCR成功地合成了AcrA、AcrB的内标准DNA。