Based on homologous recombination, a recombinant PRV co-expressing protein precursor P1-2A of FMDV and VP2 protein of PPV had been constructed using PRV TK7gE7LacZ+ mutant as the vector virus.

• 采用脂质体介导法将pIEP1-2A-3C与伪狂犬病病毒基因缺失标志疫苗株PRV TK~-/gE~-/LacZ~+基因组共转染,构建了表达口蹄疫病毒P1-2A和3C基因的重组伪狂犬病病毒TK/gE~-/P1-2A-3C,并用空斑筛选与PCR鉴定的方法纯化重组病毒。