Baculovirus anti-apoptotic genes Op-iap and p35 were cloned from pIEl-4opiap and pIEl-4p35 using PCR with three pairs of primers designed according to the encoding region sequences.

• 依目的基因编码区序列设计了三对引物,从昆虫表达载体pIE1-40piap和plE1-4p35中扩增了细胞凋亡抑制基因iap和p35。