At first, the gpd gene was cloned. In order to studying the relationship between gpd gene and V. volvacea cold shock response, the expression pattern of gpd was performed in low temperature.

• 首先，本实验通过RACE的方法克隆了草菇的gpd基因，从转录水平上探讨了草菇gpd基因在低温条件下的表达量情况，以期了解gpd基因与草菇低温应答的关系，建立了以gpd为内标基因检测草菇冷诱导相关基因在低温条件下转录水平的半定量RT-PCR方法。