Apoptosis was determined by acridine orange/ethidium bromide(AO/EB) double staining, Annexin V/PI double staining, laser scanning confocul cytometry (LSC), and flow cytometry (FCM).

• 吖啶橙/溴化乙锭(AO/EB)双染色、AnnexinV/PI双染色、共聚焦显微镜、流式细胞术检测细胞凋亡;