And purified soluble linoleate isomerase was gained by refolding of denatured LI protein bound to Ni2+-NTA, and the purified recombinant linoleate isomerase possessed biological activity(5.12 U/mg),which was 2.5 times of natural linoleate isomerase.

• 采用Ni2+-NTA柱上复性法获得有活性的纯化重组亚油酸异构酶；其比活力5.；12U/mg；是天然亚油酸异构酶比活力的2