And LipofectamineTM 2000 mediated gene transfection of p498 siRNA expression vector into COS-7 cells downregulated the mRNA and protein level of 4-1BB at 48h after transfection.

• 结果经测序分析；各m4-1BB siRNA表达载体构建成功; 其中p498 siRNA与p4-1BB双质粒转染COS-7细胞；48h后可观察到细胞表面4-1BB分子明显下调；其胞内mRNA水平也显著降低；与无关干扰组比较；差异有统计学意义(P<0.；05)。