An eukaryotic vector, which highly expresses calmodulin, was constructed by DNA recombination in vitro and transfected into NRK cells.

• 实验中运用DNA体外重组技术构建了高表达钙调素的真核载体，并将其转染到大鼠正常肾细胞（NRK）中得到钙调素高表达的稳定细胞株。