An enzymatic method was applied to determine L-lactic acid based on the reaction of L-lactic acid and nicotinamide adenine dinucleotide oxidized form(NAD) catalyzed by L-lactate dehydrogenase(L-LDH).

• 利用乳酸在氧化型烟酰胺腺嘌呤二核苷酸(NAD)存在的条件下,由乳酸脱氢酶(L-LDH)催化生成丙酮酸和还原型烟酰胺腺嘌呤二核苷酸(NADH)。