Amino acid analysis shew that the ratios of basic to acidic amino acid of GO isozyme and its 40kD acidic subunit were 0. 66 and 0. 54, respectively, implying 40kD subunit was a acidic protein, 66kD band was a basic protein.

• 用制备性SDS-PAGE法获得菜心GO同工酶的40kD亚基，并和菜心GO同工酶一起测定其氨基酸组成，该GO同工酶及40kD亚基的碱/酸性氨基酸的比例分别为0.；66和0