All of the 100 random primers screened for ISSR analysis gave clear amplification products, Twe of them amplified the polymorphic DNA in the NILs Thatcher and Lr37l6 Thatcher.

• 研究采用100个ISSR引物对小麦抗叶锈近等基因系进行了分析，其中绝大多数引物能扩增出清晰可辨的条带。