After treatment with imatinib in mononuclear cell (MNC) of CML patients and K562 in vitro, expression of DNA-PKcs mRNA was detected by RT-PCR and DNA-PKcs protein level, tyrosine phosphorylation of bcr-abl fusion protein were detected by Western blot.

• 用伊马替尼体外作用CML患者的单个核细胞(MNC)及K562细胞后,用RT-PCR、Western blot方法分别检测DNA-PKcs mRNA和DNA-PKcs蛋白表达及bcr-abl融合蛋白的酪氨酸磷酸化水平。