After treatment with PBS containing 5% normal serum for 20 mins, the sections were incubated with monoclonal Tiam -1 antibody at a dilution of 1:100 for 2 hours in a moist chamber.

• 滴加1：50封闭用正常血清室温孵育20分钟，PBS稀释一抗Tiam-1(1：100)室温孵育2小时或4℃过夜；