After transient transfection into K562 cells, real-time quantitation PCR was used to detect the expression alteration of PCGF4/Bmi-1, and growth curve was utilized to observe the alteration of growth rate of cells.

• 转染K562细胞,瞬时干扰PCGF4/Bmi-1基因的表达,实时定量PCR检测干扰前后基因表达变化。 利用生长曲线测定干扰载体转染前后细胞生长速度变化。