After the reconstructed embryos were activated by 6-DMAP + CCB + ethanol, the cleavage rates of cytoplasm injection and electrical fusion were 33.1% and 28.9%, the morula rates were 17.8% and 15.5%.

• 直接注核法和电融合法构建重组胚的卵裂率分别为 33.；1%25和 2 8