After the interaction of HL - 60 and HBMEC for 24h, ZO -1 continuous expression was changed to be uncontinuous by fluorescence detecting, which suggested that HL - 60 cells crossed BBB by opening TJ.

• HL一60与HBMEC作用24h后，经免疫荧光检测发现20一1由正常时连续的变为不连续的。 提示HL一60细胞通过打开紧密连接进而穿过血脑屏障;