After the fetal umbilical vein endothelial cells are collected,inoculate them on 96 pores plate. Then add PG cells marked with 5,6-CFDA,and add SQ of different concentration to SQ groups.

• 胎儿脐静脉内皮细胞收集后,接种在96孔板上,然后加入已标记5,6-CFDA的PG细胞,实验组分别加入不同浓度的SQ,CO2培养箱中培养60min,再加入CD34-PE与粘附的细胞孵育30min,在荧光显微镜下检测PG与内皮细胞的粘附率。