After mice were immunized with BoNTB/Hc, the spleens were harvested ,and single-chain Fv (scFv) and Fab phage antibody libraries were constructed from the immunoglobulin heavy and light chain variable region genes.

• 用重组表达并纯化的BoNTB/Hc免疫小鼠；从其脾淋巴细胞中提取全套抗体可变区基因；分别构建scFv和Fab段噬菌体抗体文库；库容分别为6.；72x10~7cfu和5