After hepatic ischemia for 15 minutes and after reperfusion for 1 hour, 3 hours and 24 hours respectively, the hepatic tissue were resected for determining the activity of ATPase and for enzyme-histochemistry dyeing.

• 肝脏缺血15min,分别于再灌注后1、3、24h取肝组织测定Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶水平,并行酶组织化学染色,观察Ca2+-ATP酶的活性变化。