Additionally, an insertion vector pM-AAT was constructed by cloning the mAAT gene into the modified pLoxP-1.4K-4.3K vector, downstream of the neo gene and upstream of the right homology arms.

• 将mAA基因序列连接到酶切位点被修饰的pLOxp·1.；4K43K A体的Mo基因后、同源右臂前，得到一个插入型打靶载体pM·AAT。