Acstract: TheLEU2 gene of the yeast Kluyveromyces lactis was disrupted by replacing a part of the coding sequence with URA3 gene of the yeast S.cerevisiae.

• 将克鲁氏乳酸酵母LEU2 基因编码区的部分序列用酿酒酵母的URA3基因替换，然后用此段转化两株克鲁氏乳酸酵母。