ABC method combined with 24 h culture (C-ABC) after incubated specimen into monolayer Vero cell for rapid diagnosis of Herpes Simplex Virus (HSV) infection was carried out in experimental rabbits with Herpes Simplex Keratitis.

• 对兔单纯疱疹病毒性角膜炎模型眼,用培养24小时联合ABC法染色(C-ABC)快速检测HSV感染,并在HSV-1感染的Vero细胞制成的抗原片上比较了ABC法、PAP法、IIF和IIE检测HSV抗原的敏感性。