A trypsin like enzyme(AGP, Mr 14kD) was purified after ammonium sulfate precipitation, Sephadex G 75 gel filtration, Sephadex DEAE A 25 ion exchange chromatography and SBBI Sepharose 4B affinity chromatography.

• 肠液经硫铵沉淀，SephadexG?75凝胶过滤，SephadexDEAEA?25离子交换和SBBI?Sepharose4B亲和层析，分离纯化出分子量约为14kD的巨尾阿丽蝇蛋白酶。