A transfering vector was constructed by inserted NDV F gene and IBDV VPO gene, under the control of two synthetic promoters ATI P7. 5X20 which were conjugated at opposite direction inserted within the thymidine kinase (TK) gene of FPV 282E4 strain.

• 将NDV F基因和IBDV VPO基因分别置于人工合成的复合启动子ATI·p7.；5×20的下游，将两个表达单元以复合启动子反向方式连接，插入TK基因中，得到两个复合启动子分别调控两个不同基因的重组转移质粒pVPO-2ATI·p7