A simple method was used to adapt refined needles for the collection of Th.intermedium 2Ai-2 chromosome carrying BYDV (barley yellow dwarf virus) resistance gene from meiosis-metaphase spreads .

• 本研究利用微细玻璃针法从减数分裂中期I的花粉母细胞中分离回收了携带小麦抗黄矮病基因的中间偃麦草染色体2Ai-2。